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PKA-dependent phosphorylation of IP3K-A at Ser119 regulates a binding affinity with EB3

Author:
Mo, Seo Jung, Cho, Yongsang, Choi, Byung-il, Lee, Dongmin, Kim, Hyun
Source:
Biochemical and biophysical research communications 2019 v.508 no.1 pp. 52-59
ISSN:
0006-291X
Subject:
binding capacity, cAMP-dependent protein kinase, microtubules, neurons, phosphorylation
Abstract:
Microtubule-associated end-binding protein 3 (EB3) accumulates asymmetrically at the tip-end of growing microtubules, providing a central platform for linking various cellular components. EB3 orchestrates microtubule dynamics and targeting, enabling diverse processes within neurons. Inositol 1, 4, 5-trisphosphate 3-kinase A (IP3K-A; also known as ITPKA) is a neuron-enriched protein that binds to microtubules by PKA-dependent manners. In this study, we found that IP3K-A binds to EB3 and their binding affinity is precisely regulated by protein kinase A (PKA)-dependent phosphorylation of IP3K-A at Ser119 (pSer119). We also revealed that the complex of IP3K-A and EB3 dissociates and reassociates rapidly during chemically induced LTP (cLTP) condition. This dynamic rearrangement of IP3K-A and EB3 complex will contribute remodeling of microtubule cytoskeleton allowing effective structural plasticity in response to synaptic stimulations.
Agid:
6225490