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Evaluation of Histological, Immunohistochemical, Clinical and Genetic Prognostic Factors Associated with the Response of Canine Mast Cell Tumours to Glucocorticotherapy

dos Santos Horta, R., Eunice Lavalle, G., Narducci Monteiro, L., Alves dos Reis, F.B.T.M., de Pádua Costa, M., Giuliano, A., Dantas Cassali, G., Dobson, J.
Journal of comparative pathology 2018 v.165 pp. 72-81
biopsy, dogs, genes, glucocorticoids, guidelines, histopathology, immunohistochemistry, mast cells, neoplasms, polymerase chain reaction, prednisolone, prednisone, retrospective studies
Mast cell tumours (MCTs) are one of the most frequent neoplasms in dogs. Glucocorticoids (GCs) are widely used in the management of this disease, although no guidelines for their use have been established. The aim of this study was to evaluate the correlation of MCT prognostic factors with measurable response to GCs. This retrospective study included 60 dogs treated with prednisone or prednisolone prior to surgical biopsy of MCT. Incisional or excisional biopsy was performed 7–14 days after initiation of GC therapy. Histopathology, immunohistochemical labelling for Ki67 and KITr, and polymerase chain reaction for the c-KIT gene were performed. Partial response occurred in 63.3% of cases (38/60), while 36.7% (22/60) did not respond. A response to GCs was correlated with lower stage of the disease, low histological grade, lower pattern of KITr expression and Ki67 score. Response to GCs was positively correlated with well-established favourable prognostic factors.