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A polysaccharide isolated from Sphallerocarpus gracilis protects PC12 cells against hydrogen peroxide-induced injury

Guo, Jie, Liu, Qin, Wang, Chengbo, Shi, Jipeng, Zhang, Ji
International journal of biological macromolecules 2019 v.129 pp. 1133-1139
apoptosis, arabinose, atomic force microscopy, bioactive properties, caspase-3, caspase-9, enzyme activity, food plants, glucose, glutathione peroxidase, hydrogen peroxide, mannose, molecular weight, nutritive value, polysaccharides, superoxide dismutase, China
Sphallerocarpus gracilis is a medicinal and edible plant in China with high nutritional value. We examined the structure and bioactivity of a new polysaccharide fraction purified from S. gracilis named SGP-1. This polysaccharide possessed an average molecular weight of 4.916 × 105 and was primarily composed of arabinose, mannose and glucose in a molar ratio of 1.08:94.54:4.38. Atomic force microscopy indicated that SGP-1 adopts a sphere-like chain conformation. Bioactivity tests showed that SGP-1 significantly protected PC12 cells from oxidative injury caused by H2O2 and enhanced superoxide dismutase and glutathione peroxidase activities. Moreover, SGP-1 reduced PC12 apoptosis by inhibiting Caspase-3 and Caspase-9 activities. These results provide a scientific basis for the further use of SGP-1 as a novel functional component in the treatment of oxidative damage-derived diseases.