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Swelling behavior and satiating effect of the gel microparticles obtained from callus cultures pectins

Günter, Elena A., Khramova, Daria S., Markov, Pavel A., Popeyko, Oxana V., Melekhin, Anatoliy K., Beloserov, Vladislav S., Martinson, Ekaterina A., Litvinets, Sergey G., Popov, Sergey V.
International journal of biological macromolecules 2019 v.123 pp. 300-307
Araceae, apples, body weight, callus culture, emulsions, food intake, gelation, gels, intestines, mice, microparticles, oral administration, pectins, satiety
Gel microparticles were prepared from pectins of campion (SVCgel) and duckweed (LMCgel) callus cultures, as well as from commercial apple pectin (APgel) by emulsion dehydration techniques with successive ionotropic gelation. The morphology and swelling behavior of the microparticles were determined after successive incubation in simulated gastric (SGF), intestinal (SIF), and colonic (SCF) fluids. Both SVCgel and LMCgel microparticles were found to swell in SGF and SIF gradually, and at oral administration decreased food intake by laboratory mice during the first 5 h of free-feeding. The SVCgel microparticles demonstrated the higher stability in SCF within 24 h than LMCgel ones. Only the SVCgel microparticles were shown to decrease food intake by 24% during the 21 h of free-feeding and decreased body weight of mice by 4% during 24 h after oral administration. The APgel microparticles lost their shape in SIF, then fully disintegrated after 0.5 h of incubation in SCF, and failed to affect food intake or mice body weight. The data obtained indicated that sustainability and swelling of the gel microparticles from the SVC pectin in the colonic fluid may provide the stronger satiating effect compared to that of the LMCgel microparticles.