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Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L.

Jiang, Yuan-yuan, Yu, Jun, Li, Ya-bo, Wang, Long, Hu, Liang, Zhang, Li, Zhou, Yong-hong
International journal of biological macromolecules 2019 v.123 pp. 531-538
Arctium lappa, antioxidant activity, fructose, galactose, glucose, in vivo studies, mannose, molecular weight, polysaccharides, raw materials, response surface methodology, roots, superoxide anion, temperature, ultrasonic treatment, ultrasonics
Polysaccharides were extracted from the roots of Arctium lappa L. (ALPs) using response surface methodology with ultrasonication. A central composition design was used to optimize extraction parameters by maximizing the polysaccharide extraction yield. The modified optimal conditions were as follows: water to raw material ratio of 31 mL/g, ultrasonic power of 158 W, extraction time of 83 min, and extraction temperature of 50 °C. Furthermore, fractions of ALP40-1, ALP60-1, and ALP80-1 were obtained for chemical and antioxidant activity analyses after purification. Results indicated that the three fractions had a molecular weight of 218, 178, and 60 kDa, respectively, and were composed of mannose, glucose, fructose, and galactose. ALP60-1 exhibited strong scavenging activities on 1,1‑diphenyl‑2‑picryhydrazyl, hydroxyl, and superoxide radicals. These results demonstrate that ultrasonic-assisted extraction is a very effective method for extracting ALPs, and ALP60-1 is a potential novel natural antioxidant. However, further structure elucidation and in vivo experiments are required.