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Development and characterization of mouse monoclonal antibodies to eight human complement components: Analysis of reactivity with orthologs of nine mammalian genera

Author:
Monedero, Marta, Infantes-Lorenzo, José A., Moreno, Inmaculada, Martín, Ana Belén, Macias, Rocio I.R., Toraño, Alfredo, Domínguez, Mercedes
Source:
Comparative immunology, microbiology, and infectious diseases 2019 v.62 pp. 7-12
ISSN:
0147-9571
Subject:
Leishmania, Western blotting, antibody specificity, complement, dogs, enzyme-linked immunosorbent assay, epitopes, flow cytometry, goats, guinea pigs, hamsters, humans, infectious diseases, mice, monoclonal antibodies, promastigotes, rabbits, rats, swine
Abstract:
To study complement function in mammalian leishmanioses, we developed mouse monoclonal antibodies to the human complement system components C1q, C4, factor D, factor H, factor B, properdin, C5 and C9. Antibody specificity was determined by indirect and capture ELISA and by Western blot. In flow cytometry analysis, seven antibodies recognized the cognate component on human serum-opsonized Leishmania promastigotes. Antibody reactivity was screened against promastigotes opsonized with sera of nine mammalian genera: pig, guinea pig, goat, rabbit, cat, dog, hamster, jird and rat. No antibody recognized jird epitopes on promastigotes. Anti-C4, -properdin, and -C5b reacted with the orthologous protein of all other mammals tested except cat (anti-properdin) and hamster (anti-C5b); anti-C9 only recognized the rabbit ortholog, and anti-C1q, -factor B and -factor H did not react with any of the nine orthologs. Such interspecies crossreactive antibodies can be valuable tools for analysis of mammalian complement function in infectious diseases.
Agid:
6227255