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Colorimetric assay for active biomass quantification of Fusarium fujikuroi

Author:
Cen, Yu-Ke, Lin, Jian-Guang, Wang, Jun-You, Liu, Zhi-Qiang, Zheng, Yu-Guo
Source:
Journal of microbiological methods 2018 v.155 pp. 37-41
ISSN:
0167-7012
Subject:
Fusarium fujikuroi, biomass, biosynthesis, carbon, cell growth, colorimetry, fermentation, gibberellins, menadione, quantitative analysis, tetrazolium
Abstract:
A colorimetric assay has been developed for quantitative analysis of active biomass of Fusarium fujikuroi, based on the reduction of the tetrazolium salt 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) when menadione was present as an electron coupling agent. The optimum assay-conditions were set as 200 μg/ml XTT, 5 μM menadione and one-hour reaction time. Under these settings, the produced formazan displayed a linear relationship with F. fujikuroi biomass. This method was subsequently applied to evaluate the cell growth behavior, which showed a positive correlation with the carbon source consumption and gibberellin biosynthesis under the industrial fermentation conditions. Our results showed that the XTT-menadione assay is a valuable tool in analyzing the industrial fermentation process of F. fujikuroi, especially when the medium contains insoluble and complex components.
Agid:
6227686