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Characterization and application of chemical-resistant polyurethane-based enzyme and whole cell compartments

Uhrich, Diana, Jang, Hyun-Young, Park, Jin-Byung, von Langermann, Jan
Journal of biotechnology 2019 v.289 pp. 31-38
alcohol dehydrogenase, catalytic activity, emulsions, enantioselectivity, esters, hydrolysis, polymers, solvents
This study presents the preparation and physical-chemical characterization of chemical resistant polyurethane-based compartments for biocatalytic application. The artificial compartments were prepared from an emulsion of polymer precursor and an aqueous phase that includes a biocatalytic reaction system. After curing, highly dispersed aqueous domains were obtained, which still contain the entire biocatalytic reaction system and remain fixed in the solid polymer preparation. The tensile and compression behavior of the prepared polymeric material is not significantly affected by the incorporation and facilitates excellent stability against various organic solvents and acid solutions. Thereby, the compartments can be used not only for enantioselective alcohol-dehydrogenase catalyzed reduction but also for a whole cell catalyzed hydrolysis of esters. Moreover, the compartmented whole-cell system was considerably stable to allow multiple reuses without a noticeable loss of catalytic activity of the incorporated whole cell catalytic reaction system.