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Molecular characterization of two novel proteins All1122 and Alr0750 of Anabaena PCC 7120 conferring tolerance to multiple abiotic stresses in Escherichia coli
- Sen, Sonia, Rai, Ruchi, Chatterjee, Antra, Rai, Shweta, Yadav, Shivam, Agrawal, Chhavi, Rai, L.C.
- Gene 2019 v.685 pp. 230-241
- Anabaena, Escherichia coli, abiotic stress, adenosine triphosphate, arsenic, cadmium chloride, drought, genomics, heat, heterologous gene expression, mannitol, mutants, nitrogen, paddies, paraquat, proteins, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, sequence alignment, sodium chloride, stress tolerance, ultraviolet radiation
- In- silico and functional genomics approaches have been used to determine cellular functions of two hypothetical proteins All1122 and Alr0750 of Anabaena sp. PCC 7120. Motif analysis and multiple sequence alignment predicted them as typical α/β ATP binding universal stress family protein-A (UspA) with G-(2×)-G-(9×)-G(S/T) as conserved motif. qRT-PCR data under UV-B, NaCl, heat, As, CdCl2, mannitol and methyl viologen registered approximately 1.4 to 4.3 fold induction of all1122 and alr0750 thus confirming their multiple abiotic stress tolerance potential. The recombinant E. coli (BL21) cells harboring All1122 and Alr0750 showed 12–41% and 23–41% better growth respectively over wild type control under said abiotic stresses thus revalidating their stress coping ability. Functional complementation on heterologous expression in UspA mutant E. coli strain LN29MG1655 (ΔuspA::Kan) attested their UspA family membership. This study tempted us to suggest that recombinant Anabaena PCC 7120 over expressing all1122 and alr0750 might contribute to the nitrogen economy in paddy fields experiencing array of abiotic stresses including drought and nutrient limitation.