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Reactive oxygen species inhibit biofilm formation of Listeria monocytogenes

Author:
Guo, Liang, Zhang, Chao, Chen, Guowei, Wu, Man, Liu, Wukang, Ding, Chengchao, Dong, Qingli, Fan, Enguo, Liu, Qing
Source:
Microbial pathogenesis 2019 v.127 pp. 183-189
ISSN:
0882-4010
Subject:
Listeria monocytogenes, NAD(P)H oxidase (H2O2-forming), acetylcysteine, bacterial growth, biofilm, enzyme inhibitors, models, reactive oxygen species
Abstract:
Although the level of reactive oxygen species (ROS) is altered upon the formation of bacterial biofilm, the relationship between ROS alteration and biofilm formation is still unclear. The aim of the present study is to use Listeria monocytogenes (L. monocytogenes) as a model organism to examine whether ROS have an effect on the biofilm formation. After eliminating ROS by treatment with NAD(P)H oxidase inhibitor Diphenyleneiodonium chloride (DPI) or scavenging reagents N-acetylcysteine (NAC), the biofilm formation of L. monocytogenes was examined. Our data demonstrate that DPI and NAC induced-reduction of ROS enhances the biofilm formation in L. monocytogenes without affecting bacterial growth and activity. These data provide the evidence that ROS produced by L. monocytogenes inhibit the biofilm formation.
Agid:
6229136