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Transcriptome analysis of Sclerotinia ginseng and comparative analysis with the genome of Sclerotinia sclerotiorum
- Wang, Dan, Fu, Junfan, Zhou, Rujun, Li, Zibo, Han, Yueling, Liu, Xinran
- Physiological and molecular plant pathology 2019 v.106 pp. 30-41
- Panax, Sclerotinia sclerotiorum, pathogens, phylogeny, polygalacturonase, sequence analysis, transcription factors, transcriptome, transcriptomics, unigenes, China
- Sclerotinia ginseng is a non-model necrotrophic pathogen responsible for a serious epidemic in the ginseng production area in northeast China. In this study, de novo RNA sequencing was carried out using an Illumina HiSeq™ 4000 platform in conjunction with the short-read assembly program Trinity. The S. ginseng transcriptome was compared with that of Sclerotinia sclerotiorum, and species-specific unigenes were annotated with KEGG and GO terms. Phylogenetic analysis of amino acid sequences for four polygalacturonase (pg) genes and a pH-response transcription factor (pac1) homologous gene identified in S. ginseng revealed a close evolutionary relationship with other species of Sclerotiniaceae.