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Bacillus glycinifermentans sp. nov., isolated from fermented soybean paste

Author:
Kim, Soo-Jin, Dunlap, Christopher A., Kwon, Soon-Wo, Rooney, Alejandro P.
Source:
International journal of systematic and evolutionary microbiology 2015 v.65 no.10 pp. 3586-3590
ISSN:
1466-5026
Subject:
Bacillus licheniformis, Bacillus sonorensis, DNA, Gram-positive bacteria, cell walls, fatty acids, fermentation, foods, galactose, genes, glycolipids, isoprenoids, maltose, menaquinones, monophyly, new species, nucleotide sequences, pH, peptidoglycans, phenotype, phosphatidylethanolamines, ribosomal RNA, sequence analysis, sodium chloride, sorbitol, soybeans, strains, trehalose
Abstract:
Two independent isolates of a Gram-positive, aerobic, motile rod-shaped bacterium were recovered from soybean-based fermented foodstuffs. Two were isolated from cheonggukjang, a Korean fermented soybean food product. Multilocus sequencing analysis of the 16S rRNA gene and 5 protein coding genes indicated that these strains were most closely related to Bacillus sonorensis KCTC-13918**T and Bacillus licheniformis DSM 13**T. In phenotypic characterization, the novel strains were found to grow between 15-55 'C and to tolerate up to 10% NaCl (w/v). Furthermore, the strains grew in media with pH 5 to 10 (optimal growth at pH 7.0–8.0). The predominant cellular fatty acids were anteiso-C15':'0 (42.35'%) and iso-C15':'0 (22.65'%). The predominant isoprenoid quinone was menaquinone 7 (MK-7). The cell-wall peptidoglycan contained meso-diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unknown glycolipid. The DNA G+C was 45.9 mol%. In addition, to forming a distinct monophyletic cluster in phylogenetic analysis of gene sequence data, the strains differed from the two most closely related species in that they did not metabolize D-maltose, D-trehalose, D-galactose or D-sorbitol. Based upon the consensus of phylogenetic and phenotypic analyses, these strains represent a novel species within the genus Bacillus, for which the name Bacillus glycinifermentans sp. nov. is proposed. The type strain is GO-13T ( = KACC 18425T = NRRL B-65291T).
Agid:
62295
Handle:
10113/62295