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Si-doping increases the adjuvant activity of hydroxyapatite nanorods

Author:
Wang, Xiupeng, Ihara, Shu, Li, Xia, Ito, Atsuo, Sogo, Yu, Watanabe, Yohei, Tsuji, Noriko M., Yamazaki, Atsushi
Source:
Colloids and surfaces 2019 v.174 pp. 300-307
ISSN:
0927-7765
Subject:
adjuvants, biocompatibility, bone marrow, colloids, cytokines, dendritic cells, hydroxyapatite, immune response, immunogenicity, nanorods, secretion, silica, silicon, surface area, vaccines
Abstract:
Recombinant protein-based vaccines generally show limited immunogenicity and need adjuvants to achieve robust immune responses. Herein, to combine the excellent biocompatibility of hydroxyapatite (HA) and exciting adjuvant activity of silica, Si-doped HA nanorods with Si/P molar ratio from 0 to 0.65 were hydrothermally synthesized and evaluated as immunoadjuvants. Si-doping decreases the size and increases the BET surface area of the nanorods. Si-doping in HA nanorods increases the in vitro adjuvant activity, including CD11c+CD86+ expression and cytokine secretion of bone marrow derived dendritic cells (BMDCs). Moreover, Si-doping in HA increases the ex vivo adjuvant activity as shown by the increase in both Th1 and Th2 cytokines secretion. Si-doped HA nanorods are promising as a new immunoadjuvant.
Agid:
6229754