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Functionalized CD133 antibody coated stent surface simultaneously promotes EPCs adhesion and inhibits smooth muscle cell proliferation–A novel approach to prevent in-stent restenosis

Wawrzyńska, Magdalena, Duda, Maciej, Wysokińska, Edyta, Strządała, Leon, Biały, Dariusz, Ulatowska-Jarża, Agnieszka, Kałas, Wojciech, Kraszewski, Sebastian, Pasławski, Robert, Biernat, Paweł, Pasławska, Urszula, Zielonka, Aleksandra, Podbielska, Halina, Kopaczyńska, Marta
Colloids and surfaces 2019 v.174 pp. 587-597
adhesion, adverse effects, animal models, antibodies, antigen-antibody reactions, biochemical compounds, cell proliferation, coatings, colloids, molecular models, myocytes, probability, risk reduction, smooth muscle, stem cells, titanium dioxide
We report a multistep strategy of biochemical surface modifications that resulted in the synthesis of new, effective and biocompatible intravascular implants coating with immobilized anti-CD133 antibodies, that proved to be the most effective in endothelial progenitor cells capture and reduced smooth muscle cells growth. Biomolecules were immobilized on differently functionalized surfaces. The distribution, nanostructural characteristics and intramolecular interactions of anti-CD133 molecules as well as their ability to bind EPCs was evaluated. We also tempted to build a molecular model of the CD133 protein to study antigen-antibody interactions. CD133 protein is expressed in endothelial progenitor cells (EPCs). Absence of preferential interaction site on CD133, but rather a presence of a small binding area, may be the specificity of reconnaissance sequence, thus importantly increasing the probability of CD133 protein binding. After all, regarding our molecular model, we are convinced that specific, and large enough interactions between anti-CD133 coating stent surface and CD133 present on EPCs will reduce risk of restenosis by favoring the endothelial growth. Additionally, the safety study of the vivo performance of modified titania based surface was performed using small animal models. No allergological or toxical local or systemic adverse effects of the developed coatings were noted.