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Biosynthesized of reduced graphene oxide nanosheets and its loading with paclitaxel for their anti cancer effect for treatment of lung cancer

Lin, Shaobing, Ruan, Junshan, Wang, Shaoming
Journal of photochemistry and photobiology 2019 v.191 pp. 13-17
Euphorbia milii, antineoplastic activity, cell viability, drug therapy, graphene oxide, human cell lines, humans, image analysis, leaf extracts, lung neoplasms, nanocarriers, nanocomposites, nanosheets, neoplasm cells, paclitaxel, toxicity testing, ultraviolet-visible spectroscopy
Multifunctional nanocarriers are in immediate need to develop anticancer activity for the treatment of cancers. In the present research, the graphene oxide was reduced via an efficient method which reduced to RGO by using Euphorbia milii leaves extract. Thus obtained RGO nanocomposites were subsequently characterized by means UV–Vis absorption technique. AFM imaging was further performed in order to analyze the surface morphology of GO nanosheet as well as to estimate the average thickness of the GO nanosheets before and after the addition of Euphorbia milii leaves extract. Furthermore, the anticancer effect of RGO-loaded PTX (RGO/PTX) on A549 (Human lung cancer cell lines) was evaluated by MTT assay. The results displayed that with the increase in the concentration of RGO/PTX to200 ??g/mL, the cell viability reduced to 29%. Even more increase in the concentration to 500 ??g/mL of RGO/PTX, the cell viability also showed rapid reduction to 10%. Based on this, we can conclude that the increased concentration of RGO/PTX decreased the cell viability of A549 cell lines tremendously and has the potential to serve in the lung carcinoma targeted chemotherapy.