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Comparative proteomic analysis of Nostoc flagelliforme reveals the difference in adaptive mechanism in response to different ultraviolet-B radiation treatments

Author:
Shen, Shi-gang, Guo, Rong-jun, Yan, Rong-rong, Wu, Yi-kai, Zhao, Dong-xue, Lin, Ya-hui, Lv, He-xin, Jia, Shi-ru, Han, Pei-pei
Source:
Molecular biology reports 2018 v.45 no.6 pp. 1995-2006
ISSN:
0301-4851
Subject:
Calvin cycle, DNA, Nostoc flagelliforme, amino acid metabolism, antioxidant activity, polysaccharides, protein synthesis, proteins, proteome, proteomics, ultraviolet radiation
Abstract:
Nostoc flagelliforme is a pioneer organism in the desert and highly resistant to ultraviolet B (UV-B) radiation, while the involved adaptive mechanism has not been fully explored yet. To elucidate the responsive mechanism, two doses of UV-B radiation (low: 1 W/m² and high: 5 W/m²) were irradiated for 6 h and 48 h, respectively, and their effects on global metabolism in N. flagelliforme were comprehensively investigated. In this study, we used iTRAQ-based proteomic approach to explore the proteomes of N. flagelliforme, and 151, 172, 124 and 148 differentially expressed proteins were identified under low and high UV-B doses for 6 h and 48 h, respectively. Functional classification analysis showed these proteins were mainly involved in photosynthesis, amino acid metabolism, antioxidant activity and carbohydrate metabolism. Further analysis revealed that UV-B imposed restrictions on primary metabolism including photosynthesis, Calvin cycle, and amino acid metabolism, and cells started defense mechanism through repair of DNA and protein damage, increasing antioxidant activity, and accumulating extracellular polysaccharides to minimize the damage. Moreover, high UV-B dose imposed more severe restrictions and activated stronger defense mechanism compared with low dose. The results would improve the understanding of molecular mechanisms of UV-B-stress adaption in N. flagelliforme.
Agid:
6231419