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Development and Characterization of MIR604 Resistance in a Western Corn Rootworm Population (Coleoptera: Chrysomelidae)

Lisa N. Meihls, Daniel L. Frank, Mark R. Ellersieck, Bruce E. Hibbard
Environmental entomology 2016 v.45 no.2 pp. 526-536
Diabrotica virgifera virgifera, adults, corn, crystal proteins, females, greenhouses, heritability, insect control, insecticidal proteins, insecticide resistance, larvae, lethal concentration 50, males, mortality, progeny, reciprocal crosses, seedlings, toxicity
mCry3A is one of only four proteins licensed for commercial use in Diabrotica control. Utilizing a colony of western corn rootworm, Diabrotica virgifera virgifera LeConte, selected for resistance to mCry3A, we evaluated how mCry3A resistance was inherited and whether fitness costs were associated with mCry3A resistance. Reciprocal crosses between a selected colony and a control colony were performed; resulting progeny along with parent colonies were evaluated in dose toxicity assays, greenhouse assays, and seedling assays. Dose toxicity assay results were inconclusive, as the highest dose of protein tested did not produce sufficient mortality for accurate LC₅₀ calculation. In whole-plant greenhouse assays on mCry3A-expressing corn, larval relative survival of the selected female × control male reciprocal cross was similar to that of the selected colony, while that of the control female × selected male was intermediate between the mCry3-selected colony and the control colony. However, when adult relative survival in whole-plant greenhouse assays was examined, no significant difference between the reciprocal crosses and the two parent colonies was detected. Heritability calculations based on both larval (0.66) and adult (1.03) survival data indicate that resistance to mCry3A is not inherited in a recessive manner. The selected colony was removed from selection pressure and evaluated after three or eight generations of removal. At three generations of removal from selection, a slight decrease in larval relative survival was detected compared with the selected colony. At eight generations of removal from selection, larval relative survival was comparable with that of the selected colony.