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Application of encapsulated magnesium peroxide (MgO2) nanoparticles in permeable reactive barrier (PRB) for naphthalene and toluene bioremediation from groundwater

Gholami, Fatemeh, Mosmeri, Hamid, Shavandi, Mahmoud, Dastgheib, Seyed Mohammad Mehdi, Amoozegar, Mohammad Ali
The Science of the total environment 2019 v.655 pp. 633-640
biodiversity, bioremediation, community structure, decontamination, encapsulation, genes, groundwater, high-throughput nucleotide sequencing, microbial communities, microorganisms, nanoparticles, naphthalene, oxygen, petroleum, polymethylmethacrylate, porous media, ribosomal RNA, scanning electron microscopy, toluene
One of the challenges in the petroleum hydrocarbon contaminated groundwater remediation by oxygen releasing compounds (ORCs) is to identify the remediation mechanism and determine the impact of ORCs on the environment and the intrinsic groundwater microorganisms. In this research, the application of encapsulated magnesium peroxide (MgO2) nanoparticles in the permeable reactive barrier (PRB) for bioremediation of the groundwater contaminated by toluene and naphthalene was studied in the continuous flow sand-packed plexiglass columns within 50 d experiments. For the biodiversity studies, next generation sequencing (NGS) of the 16S rRNA gene was applied. The results showed that naphthalene was metabolized (within 20 days) faster than toluene (after 30 days) by microorganisms of the aqueous phase. By comparing the contaminant removal in the biotic (which resulted in the complete contaminant removal) and abiotic (around 32% removal for naphthalene and 36% for toluene after 50 d) conditions, the significant role of microorganisms on the decontamination process was proved. Furthermore, the attached microbial communities on the porous media were visualized by scanning electron microscopy (SEM). Microbial community structure analysis by NGS technique revealed that the microbial species which were able to degrade toluene and naphthalene such as P. putida and P. mendocina respectively were stimulated by addition of MgO2 nanoparticles. The presented study resulted in a momentous insight into the application of MgO2 nanoparticles in the hydrocarbon compounds removal from groundwater.