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Simple, low-cost, sensitive and label-free aptasensor for the detection of cardiac troponin I based on a gold nanoparticles modified titanium foil

Lopa, Nasrin Siraj, Rahman, Md. Mahbubur, Ahmed, Faiz, Ryu, Taewook, Sutradhar, Sabuj Chandra, Lei, Jin, Kim, Jaewoong, Kim, Dae Ho, Lee, Yong Hoon, Kim, Whangi
Biosensors & bioelectronics 2019 v.126 pp. 381-388
DNA, aptasensors, blood serum, crystal structure, electrochemistry, foil, humans, nanogold, oligonucleotides, titanium, troponin I
This research demonstrated the electrochemical modification of low-cost titanium (Ti) metal substrate with gold nanoparticles (AuNPs) for the aptamer-based detection of cardiac troponin I (cTnI). AuNPs were deposited onto Ti sheets by the potential-step deposition method with high density and homogeneity as well as good crystallinity. It was then applied as a transducer to immobilize a thiol-functionalized DNA aptamer via the self-assembled monolayer mechanism for the specific binding of cTnI. This was verified through electrochemical and morphological analyses. The aptasensor could detect cTnI in a linear range of 1–1100 pM with a detection limit of ca. 0.18 pM. The aptasensor showed high sensitivity and specificity to cTnI over other interfering compounds with good recoveries in the diluted human serum samples.