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Simple, low-cost, sensitive and label-free aptasensor for the detection of cardiac troponin I based on a gold nanoparticles modified titanium foil

Author:
Lopa, Nasrin Siraj, Rahman, Md. Mahbubur, Ahmed, Faiz, Ryu, Taewook, Sutradhar, Sabuj Chandra, Lei, Jin, Kim, Jaewoong, Kim, Dae Ho, Lee, Yong Hoon, Kim, Whangi
Source:
Biosensors & bioelectronics 2019 v.126 pp. 381-388
ISSN:
0956-5663
Subject:
DNA, aptasensors, blood serum, crystal structure, electrochemistry, foil, humans, nanogold, oligonucleotides, titanium, troponin I
Abstract:
This research demonstrated the electrochemical modification of low-cost titanium (Ti) metal substrate with gold nanoparticles (AuNPs) for the aptamer-based detection of cardiac troponin I (cTnI). AuNPs were deposited onto Ti sheets by the potential-step deposition method with high density and homogeneity as well as good crystallinity. It was then applied as a transducer to immobilize a thiol-functionalized DNA aptamer via the self-assembled monolayer mechanism for the specific binding of cTnI. This was verified through electrochemical and morphological analyses. The aptasensor could detect cTnI in a linear range of 1–1100 pM with a detection limit of ca. 0.18 pM. The aptasensor showed high sensitivity and specificity to cTnI over other interfering compounds with good recoveries in the diluted human serum samples.
Agid:
6233185