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Preparation of immobilized lipase by modified polyacrylonitrile hollow membrane using nitrile-click chemistry
- Li, Youran, Wang, Hanrong, Lu, Jiawei, Chu, Alex, Zhang, Liang, Ding, Zhongyang, Xu, Sha, Gu, Zhenghua, Shi, Guiyang
- Bioresource technology 2019 v.274 pp. 9-17
- biodiesel, biomass, calcium chloride, carboxylic ester hydrolases, enzyme activity, immobilized enzymes, polyacrylonitrile, proteins, sodium alginate, transesterification
- The application of immobilized lipase in the enzymatic production of biodiesel has shown numerous advantages. In this study, surface of Polyacrylonitrile (PAN) hollow membrane was first modified using nitrile-click chemistry in order to fit for interaction with enzyme proteins. Then sodium alginate (SA) was introduced and the membrane was post-treated by CaCl2. When the prepared PAN-PEI-SA-CaCl2 was used for lipase immobilization, the protein loading was 36.90 mg/g, and the enzyme activity reached up to 54.47 U/g, which was 2.5 times as much as that of Novozym® 435. As a result, the constructed immobilized lipase obtained a maximum biodiesel yield of 78.5%, which was 2.4 times that of the Novozym® 435 in transesterification reactions. Moreover, the biodiesel yield decreased by only 11% after the immobilized enzyme was continuously used for 20 times. This study exhibits that this technic has broad application prospects in the field of conversion of biomass resources.