PubAg

Main content area

Sizing up the cell cycle: systems and quantitative approaches in Chlamydomonas

Author:
Umen, James G
Source:
Current opinion in plant biology 2018 v.46 pp. 96-103
ISSN:
1369-5266
Subject:
Chlamydomonas, algae, mitosis, models, mutants
Abstract:
The unicellular green alga Chlamydomonas provides a simplified model for defining core cell cycle functions conserved in the green lineage and for understanding multiple fission, a common cell cycle variation found in many algae. Systems-level approaches including a recent groundbreaking screen for conditional lethal cell cycle mutants and genome-wide transcriptome analyses are revealing the complex relationships among cell cycle regulators and helping define roles for CDKA/CDK1 and CDKB, the latter of which is unique to the green lineage and plays a central role in mitotic regulation. Genetic screens and quantitative single-cell analyses have provided insight into cell-size control during multiple fission including the identification of a candidate `sizer’ protein. Quantitative single-cell tracking and modeling are promising approaches for gaining additional insight into regulation of cellular and subcellular scaling during the Chlamydomonas cell cycle.
Agid:
6234242