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Investigation of graded-level soybean meal diets in red drum (Sciaenops ocellatus) using NMR-based metabolomics analysis

Casu, Fabio, Watson, Aaron M., Yost, Justin, Leffler, John W., Gaylord, T. Gibson, Barrows, Frederic T., Sandifer, Paul A., Denson, Michael R., Bearden, Daniel W.
Comparative biochemistry and physiology 2019 v.29 pp. 173-184
Sciaenops ocellatus, animal tissues, biomarkers, crude protein, experimental diets, fish, intestines, juveniles, lipids, liver, metabolome, metabolomics, muscles, nuclear magnetic resonance spectroscopy, soybean meal
We investigated changes in the metabolome in juvenile red drum (Sciaenops ocellatus) induced by increasing amounts of soybean meal (0% to 60%) in extruded, fishmeal-free diets using a nuclear magnetic resonance spectroscopy (NMR)-based metabolomics approach in a 12-week feeding trial. All of the diets were composed of ≈40% total crude protein, ≈11% total crude lipid and were energetically balanced. A fishmeal-containing, commercial extruded diet was used as a control diet throughout the trial. Each week, liver, muscle, intestine and plasma samples were collected and analyzed by NMR to provide a “snapshot” of the metabolome at different time points. Results indicate significant time-dependence of the metabolic profiles in various tissues with stable metabolomic profiles forming after about 9-weeks on the experimental diets. We identify a previously unexploited biomarker of potential dietary stress (N‑formimino‑l‑glutamate (FIGLU)) in the fish that may prove to be useful for optimization of alternative diet formulations.