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Investigation of graded-level soybean meal diets in red drum (Sciaenops ocellatus) using NMR-based metabolomics analysis

Author:
Casu, Fabio, Watson, Aaron M., Yost, Justin, Leffler, John W., Gaylord, T. Gibson, Barrows, Frederic T., Sandifer, Paul A., Denson, Michael R., Bearden, Daniel W.
Source:
Comparative biochemistry and physiology 2019 v.29 pp. 173-184
ISSN:
1744-117X
Subject:
Sciaenops ocellatus, animal tissues, biomarkers, crude protein, experimental diets, fish, intestines, juveniles, lipids, liver, metabolome, metabolomics, muscles, nuclear magnetic resonance spectroscopy, soybean meal
Abstract:
We investigated changes in the metabolome in juvenile red drum (Sciaenops ocellatus) induced by increasing amounts of soybean meal (0% to 60%) in extruded, fishmeal-free diets using a nuclear magnetic resonance spectroscopy (NMR)-based metabolomics approach in a 12-week feeding trial. All of the diets were composed of ≈40% total crude protein, ≈11% total crude lipid and were energetically balanced. A fishmeal-containing, commercial extruded diet was used as a control diet throughout the trial. Each week, liver, muscle, intestine and plasma samples were collected and analyzed by NMR to provide a “snapshot” of the metabolome at different time points. Results indicate significant time-dependence of the metabolic profiles in various tissues with stable metabolomic profiles forming after about 9-weeks on the experimental diets. We identify a previously unexploited biomarker of potential dietary stress (N‑formimino‑l‑glutamate (FIGLU)) in the fish that may prove to be useful for optimization of alternative diet formulations.
Agid:
6234805