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Arginine supplementation reduces colonic injury, inflammation and oxidative stress of DSS-induced colitis in mice

Andrade, Maria Emília Rabelo, Barros, Patrícia Aparecida Vieira de, Menta, Penélope Lacrísio dos Reis, Costa, Grazielle Mara Ferreira, Miranda, Sued Eustáquio Mendes, Leocádio, Paola Caroline Lacerda, Almeida-Leite, Camila Megale de, Generoso, Simone de Vasconcelos, Leite, Jacqueline Isaura Alvarez, Cardoso, Valbert Nascimento
Journal of functional foods 2019 v.52 pp. 360-369
arginine, colitis, collagen, colon, cytokines, diet, histology, immunoglobulin A, inducible nitric oxide synthase, inflammation, mice, models, morphometry, oxidative stress, permeability, therapeutics, tight junctions
This study evaluated the mechanisms action of arginine in experimental model of ulcerative colitis. C57BL/6 mice were randomized into 4 groups: Control group (C): standard diet; Colitis group (Col): standard diet and DSS solution; Pretreated group (PT): diet supplementation with arginine before and during colitis induction; Treated group (T): diet supplementation with arginine during colitis induction. Colitis was induced by administration of 1.5% DSS for 5 days. Parameters such as intestinal permeability (IP), bacterial translocation (BT), histological and morphometric analysis, cytokines, immunoglobulin A, inflammatory infiltrate, oxidative stress, tight junction, iNOS1Nitric oxide indutase.1 were performed. The Col group showed increased IP, BT, oxidative stress and inflammatory infiltrate in the colon. Arginine decreased IP, BT, oxidative stress and inflammatory infiltrate in the colon. Only, the group T reduced iNOS, cytokine IL-17,2Interleukine 17.2 and increased collagen area and cytokine TGF-β.3Transforming growth factor beta.3 These results suggest that arginine can constitute in potential therapy for colitis.