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Optimization of fibrinolytic enzyme production by newly isolated Bacillus subtilis Egy using central composite design

Author:
Moharam, Maysa E., El-Bendary, Magda A., El-Beih, Fawkia, Hassanin Easa, Saadia M., Abo Elsoud, Mostafa M., Azzam, Mohamed I., Elgamal, Nora N.
Source:
Biocatalysis and agricultural biotechnology 2019 v.17 pp. 43-50
ISSN:
1878-8181
Subject:
Bacillus subtilis, aeration, cost effectiveness, culture media, enzyme activity, forage, inoculum, models, pH, ribosomal DNA, sequence analysis, temperature, yeast extract, yeasts
Abstract:
In the present study a fibrinolytic enzyme producer was isolated and identified as Bacillus subtilis using 16S rDNA sequencing. Central Composite Design was used for optimization of enzyme production using fodder yeast as a cost effective growth medium. The obtained results revealed that fodder yeast concentration, incubation temperature, aeration level followed by yeast extract concentration and incubation period are significant factors affect the enzyme production yield by the tested organism. Optimum levels of the selected variables were 3.05% fodder yeast, 0.71% yeast extract, initial pH 7,20% aeration level, 3.2% inoculum size (16 × 106CFU), 36.7 °C incubation temperature and 4 days incubation period. At these conditions the predicted enzyme activity was 18.9 U/ml and the practical enzyme activity was 16.6 U/ml which revealed that the model was valid by 87.83%. The results were discussed in the light of possible application as a thrombolytic agent.