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Antioxidant peptide-loaded electrospun chitosan/poly(vinyl alcohol) nanofibrous mat intended for food biopackaging purposes

Author:
Hosseini, Seyed Fakhreddin, Nahvi, Zahra, Zandi, Mojgan
Source:
Food hydrocolloids 2019 v.89 pp. 637-648
ISSN:
0268-005X
Subject:
Fourier transform infrared spectroscopy, X-ray diffraction, antioxidant activity, antioxidants, atomic force microscopy, bio-packaging, cell lines, chitosan, contact angle, cytotoxicity, electric potential difference, encapsulation, hydrocolloids, hydrophobicity, moieties, nanofibers, packaging materials, peptides, permeability, polyvinyl alcohol, surface roughness, tensile strength, thermal stability, water vapor
Abstract:
Electrospun chitosan/poly(vinyl alcohol) (CS/PVA) fibrous mats encapsulating fish-purified antioxidant peptide (AOP) were successfully fabricated under the optimal conditions (flow rate = 0.2 mL/h, applied voltage = 15 kV, and tip-to-collector distance = 15 cm). The AOP-loaded electrospun fibers displayed uniform and bead-free nanostructures with fiber diameter ranging from 157.9 ± 28.8 to 195.5 ± 34.4 nm, indicating the miscibility of CS and PVA polymers in nanoscale. Atomic force microscopy revealed the dense fibrous nature of the mats, with surface roughness ranging from 180.6 to 279.1 nm. Fourier transform infrared spectroscopy and X-ray diffraction analyses demonstrated the molecular interaction between components functional groups, which enhanced the mats thermal stability and mechanical properties (e.g. around 3-fold, in terms of tensile strength) in contrast to pure PVA. Water vapor permeability of electrospun nanofibers decreased with the inclusion of CS and/or peptide into fibrous matrices. Moreover, water contact angle analysis showed that the incorporation of AOP made the blend nanofibrous mat more hydrophobic, in which the highest value achieved was 109.45 ± 3.88° at θt=0. Importantly, CS/PVA/AOP ternary fibers showed high encapsulation efficiency beyond 94%, and the encapsulated peptide retained its antioxidant activity in electrospun fibers. The loading of the peptide within nanofibers enables a sustained release of AOP. Also, in vitro cell toxicity experiment which was performed by MTT method on L929 cell lines displayed good cytocompatibility of the AOP-loaded nanofibers. The study provides a facile approach for developing fiber-based bioactive packaging materials of interest in food biopackaging purposes.
Agid:
6235926