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Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov.
- Du, Zong-Jun, Wang, Ying, Dunlap, Christopher, Rooney, Alejandro P., Chen, Guan-Jun
- International journal of systematic and evolutionary microbiology 2014 v.64 no.5 pp. 1690
- DNA, Gram-negative bacteria, fatty acids, genome, menaquinones, microbial growth, new family, new genus, new species, nucleic acid hybridization, nucleotide sequences, pH, phosphatidylethanolamines, ribosomal RNA, sequence homology, sodium chloride, strains
- The taxonomic characteristics of two bacterial strains, FH5T and SS4, isolated from enrichment cultures obtained from two distinct marine environments, were determined. These bacteria were Gram-stain-negative, facultatively anaerobic rods. Growth occurred at 20–40 °C (optimum, 28– 32 °C), pH 5.5–9.0 (optimum, pH 7.0–7.5) and in the presence of 1–7% NaCl (optimum, 2– 4 %). The major cellular fatty acids were anteiso-C(15:0) and iso-C(15:0). Menaquinone 7 (MK-7) was the sole respiratory quinone. The major polar lipids were phosphatidylethanolamine, an unkown phospholipid and an unknown lipid. The DNA G+C contents of strains FH5(T) and SS4 were both determined to be 42.0 mol%. The results of DNA–DNA hybridization studies indicated that the FH5(T) and SS4 genomes share greater than 95% relatedness. The strains formed a distinct phyletic line within the class Bacteroidia, with less than 89.4% sequence similarity to their closest relatives with validly published names. On the basis of physiological and biochemical characteristics, 16S rRNA gene sequences and chemical properties, a novel genus and species, Draconibacterium orientale gen. nov., sp. nov., within the class Bacteroidia, are proposed, with strain FH5T (=DSM 25947(T)=CICC 10585(T)) as the type strain. In addition, a new family, Draconibacteriaceae fam. nov., is proposed to accommodate Draconibacterium gen. nov.