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Behavior of 3-isobutyl-2-methoxypyrazine biosynthesis related to proposed precursor and intermediate in wine grape
- Lei, Yujuan, Xie, Sha, Chen, Huangzhao, Guan, Xueqiang, Zhang, Zhenwen
- Food chemistry 2019 v.277 pp. 609-616
- biochemical pathways, biosynthesis, flavor compounds, genes, methyltransferases, pyrazines, small fruits, wine grapes, wines
- Although 3-alkyl-2-methoxypyrazines (MPs) are very important flavor compounds in grape and wine, the metabolic pathway of these compounds has not been fully elucidated, and only a few studies have focused on it to date. In this study, we performed in situ incorporation of the proposed precursor (l-Leu) and the key intermediate (2-amino-4-methylpentanamide; AMPA) of 3-isobutyl-2-methoxypyrazine (IBMP) biosynthesis into Cabernet Sauvignon clusters. The IBMP content and the expression levels of key related genes were monitored. IBMP content decreased shortly after l-Leu or AMPA treatment, but subsequently increased significantly, with raised levels of O-methyltransferase genes (VvOMT1 and VvOMT3) in the berries between veraison and maturation. We speculate that l-Leu may be a precursor of IBMP biosynthesis and that AMPA may be a key intermediate.