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A novel strategy for isolation and purification of fucoxanthinol and fucoxanthin from the diatom Nitzschia laevis
- Sun, Peipei, Wong, Chi-Chun, Li, Yuelian, He, Yongjin, Mao, Xuemei, Wu, Tao, Ren, Yuanyuan, Chen, Feng
- Food chemistry 2019 v.277 pp. 566-572
- Nitzschia, carbon, carotenoids, cost effectiveness, mass spectrometry, microalgae, nuclear magnetic resonance spectroscopy, solid phase extraction, stable isotopes, thin layer chromatography, ultra-performance liquid chromatography
- In this study, the microalga Nitzschia laevis (N. laevis) can accumulate a marine carotenoid fucoxanthinol. In particular, fucoxanthinol was firstly isolated from microalgae, accompanied by its derivative fucoxanthin. The identification and quantification of fucoxanthinol and fucoxanthin were determined by ultra-performance liquid chromatography coupled to photodiode array detector-quadrupole/travelling-wave ion mobility mass spectrometry/time-of-flight mass spectrometry (UPLC-PDA-TWIMS-QTOF-MS). Furthermore, a cost-effective approach mediated with solid-phase extraction (SPE) and thin-layer chromatography (TLC) technique was used to isolate and purify fucoxanthinol and fucoxanthin from the extracts of N. laevis. This two-step method can obtain 98% fucoxanthinol and 95% fucoxanthin, with the recovery efficiencies of around 85% for fucoxanthinol and 70% for fucoxanthin, respectively. Moreover, 1H and 13C nuclear magnetic resonance (NMR) techniques were adopted to record the purified compounds for supporting the above results. In all, the developed method has a promising potential to purify fucoxanthinol and fucoxanthin of microalgae for food and pharmaceutical applications.