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A novel SERS-based aptasensor for ultrasensitive sensing of microcystin-LR

Author:
He, Deyun, Wu, Zhengzong, Cui, Bo, Jin, Zhengyu
Source:
Food chemistry 2019 v.278 pp. 197-202
ISSN:
0308-8146
Subject:
DNA fragmentation, Raman spectroscopy, aptasensors, complementary DNA, detection limit, magnetism, microcystin-LR, nanogold, nanoparticles, oligonucleotides, tap water
Abstract:
We developed a novel aptasensor based on surface enhanced Raman spectroscopy (SERS) and applied it for highly sensitive detection of microcystin-LR (MC-LR). In this work, MC-LR aptamer and its corresponding complementary DNA fragments (cDNA) were conjugated to gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs), respectively. Then, MC-LR aptamer-AuNPs and cDNA-MNPs conjugates were used as signal probes and capture probes, respectively. The proposed assay exhibited a linearity range from 0.01 to 200 ng/mL with the limit of detection (LOD) of 0.002 ng/mL. In addition, the reliability of the novel approach was validated by detecting different levels of MC-LR spiked in tap water samples. Overall, the novel aptasensor paves a new way for rapid and accurate determination of MC-LR and can be referred to detect other hazardous substances in water products.
Agid:
6236994