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Antiobesity Effect of Prebiotic Polyphenol-Rich Grape Seed Flour Supplemented with Probiotic Kefir-Derived Lactic Acid Bacteria

Cho, Yun-Ju, Lee, Hyeon Gyu, Seo, Kun-Ho, Yokoyama, Wallace, Kim, Hyunsook
Journal of agricultural and food chemistry 2018 v.66 no.47 pp. 12498-12511
adipocytes, adipose tissue, blood lipids, body weight changes, cecum, cellulose, cholesterol, flour, gene expression, genes, glucose, grape seeds, high fat diet, immunologic diseases, inflammasomes, inflammation, insulin, insulin resistance, kefir, lactic acid bacteria, lipid content, liver, metabolic diseases, mice, microarray technology, obesity, oral administration, permeability, prebiotics, probiotics, propionic acid, synergism, weight gain, wine grapes
The interaction between prebiotics and probiotics may exert synergistic health benefits. This study investigated the combined effects of polyphenol-rich wine grape seed flour (GSF), a prebiotic, and lactic acid bacteria (LAB) derived from kefir, a probiotic, on obesity-related metabolic disease in high-fat diet (HFD) induced obese (DIO) mice. DIO mice were fed with HFD with 6% microcrystalline cellulose (CON) or HFD supplemented with GSF (5% or 10% GSF), HFD with LAB orally administrated (LAB), or HFD with a combination of GSF and LAB orally administrated (GSF+LAB) for 9 weeks. The vehicle, saline, was also orally administered to the CON and GSF groups. In comparison to CON, all GSF and LAB groups showed a reduction (P < 0.05) in HF-induced weight gain, liver and adipose tissue weights, plasma lipid concentrations, insulin resistance, and glucose intolerance. The combination of 10% GSF and LAB showed synergistic effects (P < 0.05) on body weight gain, plasma insulin and total cholesterol concentrations, and cecum propionate contents. Plasma zonulin and cecum propionate concentrations and intestinal FXR gene expression were (P < 0.05) correlated with body weight gain. A pathway analysis of microarray data of adipose tissue showed that the combination of GSF and LAB affected genes involved in metabolic and immunological diseases, including inflammasome complex assembly (P < 0.05). In conclusion, a combination of GSF and LAB inhibited HF-induced obesity and inflammation via alterations in intestinal permeability and adipocyte gene expression.