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Rapid detectoin method for fusarid acid-producing species of Fusarium by PCR

Author:
Lee, Theresa, Kim, Sosoo, Busman, Mark, Proctor, Robert H., Ham, Hyeonhui, Lee, Soohyung, Hong, Sung Kee, Ryu, Jae-Gee
Source:
Singmulbyeong yeon-gu 2015 v.21 no.4 pp. 326-329
ISSN:
1598-2262
Subject:
Fusarium circinatum, Fusarium fujikuroi, Fusarium oxysporum, Fusarium proliferatum, Fusarium tricinctum, Fusarium verticillioides, fumonisins, fungi, fusaric acid, microbial detection, multigene family, polymerase chain reaction, rapid methods, transcription factors, trichothecenes
Abstract:
Fusaric acid is a mycotoxin produced by species of the fungus Fusarium and can act synergistically with other Fusarium toxins. In order to develop a specific detection method for fusaric acid-producing fungus, PCR primĀ¬ers were designed to amplify FUB10, a transcription factor gene in fusaric acid biosynthetic gene cluster. When PCR with Fub10-f and Fub10-r was performed, a single band (~550 bp) was amplified from F. oxysporum, F. proliferatum, F. verticillioides, F. anthophilum, F. bulbicola, F. circinatum, F. fujikuroi, F. redolens, F. sacchari, F. subĀ¬glutinans, and F. thapsinum, all of which were known for fusaric acid production. Whereas the FUB10 specific band was not amplified from Fusarium species known to be trichothecene producer. Because production of fusaric acid can co-occur in species that also produce fumonisin mycotoxins, we developed a multiplex PCR assay using the FUB10 primers as well as primers for the fumonisin biosynthetic gene FUM1. The assay yielded amplicons from fumonisin producers such as F. proliferatum and F. verticillioides, allowing for the simultaneous detection of species with the genetic potential to produce both types of mycotoxins.