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A new variant of Croton yellow vein mosaic virus naturally infecting wild sunflower in India

Author:
Sharma, Renuka, Chandel, Vanita, Rishi, Narayan
Source:
Virusdisease 2018 v.29 no.4 pp. 513-519
ISSN:
2347-3584
Subject:
Croton yellow vein mosaic virus, Helianthus annuus, Papaya leaf curl virus, Verbesina encelioides, coat proteins, genome, nucleotides, polymerase chain reaction, sequence analysis, viruses, India
Abstract:
During 2015–2016, wild sunflower (Verbesina encelioides) was observed with bright yellow vein mosaic symptims in Noida, Uttar Pradesh, India. The initial analysis by PCR with a pair of coat protein based primers revealed the association of a begomovirus. Further, the virus was identified by rolling circle amplification and cloning of the complete genome. The DNA-A (2671 nucleotides, MH359168) with the genome organisation typical of the Old World begomovirus shared 98% sequence identity with that of Croton yellow vein mosaic virus (CYVMV) reported from India. The betasatellite (MH359169) associated with the disease shared 92% sequence identity with papaya leaf curl betasatellite (KY825245). The results showed that the wild sunflower is a new alternate host of CYVMV. The study also revealed a natural association of heterologous betasatellite with CYVMV in wild sunflower exhibiting yellow vein mosaic symptoms.
Agid:
6239266