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Preussia sp. BSL-10 producing nitric oxide, gibberellins, and indole acetic acid and improving rice plant growth

Author:
Al-Hosni, Khdija, Shahzad, Raheem, Latif Khan, Abdul, Muhammad Imran, Qari, Al Harrasi, Ahmed, Al Rawahi, Ahmed, Asaf, Sajjad, Kang, Sang-Mo, Yun, Byung-Wook, Lee, In-Jung
Source:
Journal of plant interactions 2018 v.13 no.1 pp. 112-118
ISSN:
1742-9153
Subject:
Preussia, aldehyde dehydrogenase, chromatography, crops, cultivars, cytochrome P-450, endophytes, gene expression, geranylgeranyl diphosphate synthase, gibberellins, hydrolases, indole acetic acid, mutants, nitrate reductase, nitric oxide, nitrite reductase, plant growth, reverse transcriptase polymerase chain reaction, rice, spectroscopy, tryptophan synthase
Abstract:
Preussia sp. have been least known to improve plant growth and produce phytohormones. The current study investigated the production of nitric oxide (NO), indole-3-acetic acid (IAA), and gibberellins (GA₄, GA₇, GA₁₅, and GA₅₃) by a novel endophytic-fungal strain Preussia sp. BSL-10 using advanced chromatographic and spectroscopic techniques. Production of these phytohormones were validated by RT–PCR analysis, which indicated the expression of genes encoding tryptophan synthase (TRP), indole-3-acetamide hydrolase (IAAH), tryptophan-2-monooxygenase (IAAM), aldehyde dehydrogenase (ALD), GA₄ desaturase (DES), geranylgeranyl-diphosphate synthase (GGS2), ent-desaturase oxidase (P450-4), GA₁₄ synthase (P450-1) and nitrite reductase (NIRK/NIRS), cytochrome P450 (P450nor), nitrate reductase (NR), NOS-like (NOL), and nitric oxide reductase (QNOR/CNOR). In plant growth-promoting effects, the inoculation of Preussia sp. BSL-10 significantly increased the growth of dwarf mutant Waito-C and wild-type rice cultivars. In conclusion, utilizing new endophytic with the ability to produce NO, IAA, and gibberellins can be used to promote growth and yield of marginalized crops.
Agid:
6239455