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Activity of defense related enzymes and gene expression in pigeon pea (Cajanus cajan) due to feeding of Helicoverpa armigera larvae

Meitei, Aheibam Loyanganba, Bhattacharjee, Mamta, Dhar, Santanu, Chowdhury, Naimisha, Sharma, Richa, Acharjee, Sumita, Sarmah, Bidyut Kumar
Journal of plant interactions 2018 v.13 no.1 pp. 231-238
Cajanus cajan, Helicoverpa armigera, cultivars, enzymes, gene expression, gene expression regulation, genes, hydrogen peroxide, larvae, pathogenesis-related proteins, pigeon peas, seed storage, trypsin inhibitors
The biochemical and molecular basis of the defense in a mild tolerant (ICPL-332) and susceptible (ICPL-87) cultivars of pigeon pea (Cajanus cajan) due to Helicoverpa. armigera infestation was studied. We found that feeding by the larvae generated H₂O₂ in a localized manner and activity was observed upto 12 h (hrs) of with a sharp decline within 24 h. Similarly, PPO activity was also detected till 12 h of treatment, which decreased after 24 h of feeding by larvae. The activity of trypsin inhibitor was detected in all the treatments when assayed at 12 and 24 h after larval feeding. The expression of defense genes like the Pre-hevein-like protein PR-4 precursor (PR-4), protease inhibitor/seed storage/LTP family protein (Ltp) were significantly up-regulated in ICPL-332 upon infestation after 12 h as compared to ICPL-87, whereas the endo 1, 4 -β-glucanase (Kor-1) gene was expressed in both the cultivars after 24 h of infestation. Both the cultivars varied with respect to the induction of defense-related genes during larval feeding, both the PR-4 and Ltp genes appeared to be important for defense against H. armigera in pigeon pea. Thus, the present study revealed an insight of herbivore-induced biochemical and molecular changes in pigeon pea.