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A novel fluorescent probe for Hg²⁺ detection in a wide pH range and its application in living cell imaging

Author:
Xu, Jing, Li, Honglin, Chen, Yanchi, Yang, Bing, Jiao, Qingcai, Yang, Yushun, Zhu, Hai-Liang
Source:
Analytical methods 2018 v.10 no.46 pp. 5554-5558
ISSN:
1759-9679
Subject:
amino acids, anions, fluorescence, fluorescent dyes, image analysis, mass spectrometry, mercury, metal ions, monitoring, nuclear magnetic resonance spectroscopy, pH
Abstract:
A novel fluorescent probe (XL-1) exhibited over 50-fold enhanced fluorescence in the presence of Hg²⁺ and no interference could be produced by other metal ions, anions and amino acids. The recognition mechanism was confirmed by NMR and HRMS. The most attractive point was that XL-1 could maintain a stable detection performance for Hg²⁺ in a wide pH range from 3.0 to 11.0, indicating that it is an effective tool for Hg²⁺ detection in different acid–base environments. Furthermore, the confocal fluorescence images demonstrated that XL-1 could be used for monitoring Hg²⁺ in living cells.
Agid:
6240333