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Zinc clasp-based reversible toolset for selective metal-mediated protein heterodimerization

Author:
Kocyła, Anna, Krężel, Artur
Source:
Chemical communications 2018 v.54 no.96 pp. 13539-13542
ISSN:
1364-548X
Subject:
dimerization, enzymes, proteins, zinc
Abstract:
Considering the complex biological quandaries of the tightly woven networks of biological macromolecules, we present an optimized zinc clasp-based toolset from the CD4 co-receptor and Lck protein tyrosine kinase complex for selective, tight and fully reversible protein heterodimerization (log K₁₂ = 18.6). We demonstrated its utility on CD4-tagged proteins with capture from bacterial lysate and constructed molecular baits using a new small-molecule tether.