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Genetic Diversity of Salmonella and Listeria Isolates from Food Facilities

Wang, Yu, Pettengill, James B., Pightling, Arthur, Timme, Ruth, Allard, Marc, Strain, Errol, Rand, Hugh
Journal of food protection 2018 v.81 no.12 pp. 2082-2089
Food and Drug Administration, Listeria, Salmonella, decision making, farms, food pathogens, genetic distance, genetic similarity, genetic variation, information sources, packing houses, population structure, probability, public health, sequence analysis, single nucleotide polymorphism, supply chain
Food production–related facilities (farms, packing houses, etc.) are monitored for foodborne pathogens, and data from these facilities can provide a rich source of information about the population structure and genetic diversity of Salmonella and Listeria. This information is of both academic interest for understanding the evolutionary forces acting on these organisms and of practical interest to those responsible for controlling pathogens in facilities and to those analyzing data from facilities in the context of public health decision making. We have collected information about all positive isolates from facility inspections performed by the U.S. Food and Drug Administration for which whole genome sequencing data are available. The within- and between-facilities observed genetic diversity of isolates was computed and related to the common origin of isolates (as the common collected facility). This relationship provides quantification for assessing the relationship between isolates based on their genetic similarity quantified by single-nucleotide polymorphisms (SNPs). Our results show that if the genetic distance (D) between two isolates is low, then more likely than not they are from the same facility or have some overlap in their supply chain. For example, if the genetic distance is no more than 20 SNPs, the probability (P) that two isolates come from the same facility = 0.66 for Salmonella and 0.70 for Listeria. However, if two isolates come from different facilities, their genetic distance is likely large (for Salmonella, P(D > 20 SNPs) = 0.99982; for Listeria, P(D > 20 SNPs) = 0.99949); even if two isolates come from the same facility, their genetic distance is also very likely large (for Salmonella, P(D > 20 SNPs) = 0.794; for Listeria, P(D > 20 SNPs) = 0.692). These results provide insight into what SNP thresholds might be appropriate when determining whether two isolates are from the same facility and thus would be of interest to those investigating foodborne outbreaks and conducting traceback investigations.