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Application of Concanavalin A–Linked Magnetic Beads for the Detection of Hepatitis A Virus

Author:
Ko, Sang-Mu, Cho, Se-Young, Oh, Myung-Joo, Kwon, Joseph, Vaidya, Bipin, Kim, Duwoon
Source:
Journal of food protection 2018 v.81 no.12 pp. 1997-2002
ISSN:
0362-028X
Subject:
Hepatitis A virus, agglutinins, binding capacity, carbohydrate binding, concanavalin A, hepatitis, immunomagnetic separation, magnetism, minimally processed foods, reverse transcriptase polymerase chain reaction, soybeans, tissue culture, viruses, wheat germ
Abstract:
Prompt and inexpensive detection of hepatitis A virus (HAV) is essential to control acute hepatitis outbreaks associated with the consumption of contaminated raw or minimally processed food. In this study, various carbohydrate-binding lectins, including concanavalin A (Con A), wheat germ agglutinin, and soybean agglutinin, were compared for their binding affinity to HAV. Con A, which showed significantly higher binding affinity than other lectins, was used to develop an alternative and affordable method to conventional antibody-linked immunomagnetic separation prior to detection of HAV using reverse transcriptase PCR. This method, Con A–linked immunomagnetic separation combined with reverse transcriptase PCR, can detect HAV at a dilution concentration of 10(−4) of the virus stock (titer: 10(4) median tissue culture infective dose per mL), indicating that Con A could be a promising candidate for concentrating HAV.
Agid:
6240806