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Improved characterization of Clematis based on new chloroplast microsatellite markers and nuclear ITS sequences

Author:
Liu, Zhigao, Korpelainen, Helena
Source:
Horticulture, environment and biotechnology 2018 v.59 no.6 pp. 889-897
ISSN:
2211-3452
Subject:
Clematis terniflora, chloroplast genome, chloroplasts, cultivar identification, cultivars, genetic analysis, genetic markers, internal transcribed spacers, loci, microsatellite repeats, oligodeoxyribonucleotides
Abstract:
Currently, there is a lack of genetic markers capable of effectively detecting polymorphisms in Clematis. Therefore, we developed new markers to investigate inter- and intraspecific diversity in Clematis. Based on the complete chloroplast genome of Clematis terniflora, simple sequence repeats were explored and primer pairs were designed for all ten adequate repeat regions (cpSSRs), which were tested in 43 individuals of 11 Clematis species. In addition, the nuclear ITS region was sequenced in 11 Clematis species. Seven cpSSR loci were found to be polymorphic in the genus and serve as markers that can distinguish different species and be used in different genetic analyses, including cultivar identification to assist the breeding of new ornamental cultivars.
Agid:
6240899