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Auxin homeostasis and signaling alterations result in the aberrant phenotype in scl mutant of cotton (Gossypium hirsutum L.)

Zhang, Jingxia, Gao, Yang, Zhang, Chuanyun, Liu, Guodong, Chen, Yu, Wang, Furong, Zhang, Jun
Revista brasileira de botânica 2018 v.41 no.4 pp. 775-784
Gossypium hirsutum, RNA transport, apical meristems, autotrophs, biosynthesis, carbon dioxide fixation, cotton, cultivars, databases, flavonoids, gene expression regulation, genes, homeostasis, indole acetic acid, leaf development, leaves, mutants, phenotype, photosynthesis, proteolysis, shoot tips, signal transduction, tandem mass spectrometry, ultra-performance liquid chromatography, China
Leaves are the most important organs for photosynthesis. Many cotton leaf mutants (Gossypium spp.) have been reported and broadened the insights of leaf development. A natural occurring small, crinkled leaf (scl) mutant of upland cotton (Gossypium hirsutum L.) occurs in SY321 (wild type, WT), a high-yield upland cotton cultivar that is widely planted in China. The scl has a small and crinkled leaf phenotype. The shoot apical meristem (SAM) of scl did not form the essential typical layered structure, and the cell layers were arranged in a disordered manner. The free IAA concentration of shoot tips was significantly reduced in the scl according to ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis. Gene expression profiles showed that 432 genes were differentially expressed between scl and WT. The top 20 enriched pathways were analyzed using the Kyoto encyclopedia of genes and genomes (KEGG) database and four pathways, including flavonoid biosynthesis, photosynthesis, RNA transport, and carbon fixation in photosynthetic organisms, were considered as significantly enriched pathways. Furthermore, a number of differentially expressed genes related to auxin activity, including flavonoids biosynthesis, auxin perception and signal transduction pathway, auxin transport, and ubiquitin-mediated proteolysis pathways, were identified, and the altered expressions of these genes might be responsible for the aberrant phenotype of the scl.