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Reflux aspiration in lungs of dogs with respiratory disease and in healthy West Highland White Terriers

Author:
Määttä, O. L. Merita, Laurila, Henna P., Holopainen, Saila, Lilja‐Maula, Liisa, Melamies, Marika, Viitanen, Sanna J., Johnson, L. R., Koho, Ninna, Neuvonen, Mikko, Niemi, Mikko, Rajamäki, Minna M.
Source:
Journal of veterinary internal medicine 2018 v.32 no.6 pp. 2074-2081
ISSN:
0891-6640
Subject:
Beagle, Irish Wolfhound, bacterial pneumonia, bile acids, bronchitis, dog diseases, dogs, fibrosis, gastric juice, gastroesophageal reflux, humans, larynx, lungs, mass spectrometry, observational studies, terriers
Abstract:
BACKGROUND: Gastroesophageal reflux and microaspiration (MA) of gastric juice are associated with various human respiratory diseases but not in dogs. OBJECTIVE: To detect the presence of bile acids in bronchoalveolar lavage fluid (BALF) of dogs with various respiratory diseases. ANIMALS: Twenty‐seven West Highland White Terriers (WHWTs) with canine idiopathic pulmonary fibrosis (CIPF), 11 dogs with bacterial pneumonia (BP), 13 with chronic bronchitis (CB), 9 with eosinophilic bronchopneumopathy (EBP), 19 with laryngeal dysfunction (LD), 8 Irish Wolfhounds (IWHs) with previous BPs, 13 healthy WHWTs, all privately owned dogs, and 6 healthy research colony Beagles METHODS: Prospective cross‐sectional observational study with convenience sampling of dogs. Bile acids were measured by mass spectrometry in BALF samples. Total bile acid (TBA) concentration was calculated as a sum of 17 different bile acids. RESULTS: Concentrations of TBA were above the limit of quantification in 78% of CIPF, 45% of BP, 62% of CB, 44% of EBP, 68% of LD, and 13% of IWH dogs. In healthy dogs, bile acids were detected less commonly in Beagles (0/6) than in healthy WHWTs (10/13). Concentrations of TBA were significantly higher in CIPF (median 0.013 μM, range not quantifiable [n.q.]‐0.14 μM, P < .001), healthy WHWTs (0.0052 μM, n.q.‐1.2 μM, P = .003), LD (0.010 μM, n.q.‐2.3 μM, P = .015), and CB (0.0078 μM, n.q.‐0.073 μM, P = .018) groups compared to Beagles (0 μM, n.q.). CONCLUSION AND CLINICAL IMPORTANCE: These results suggest that MA occurs in various respiratory diseases of dogs and also in healthy WHWTs.
Agid:
6241763