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The 'normalization' of germ-free rabbits with host-specific caecal microflora

Boot, R., Koopman, J. P., Kruijt, B. C., Lammers, R. M., Kennis, Henriëtte M., Lankhorst, A., Mullink, J. W. M. A., Stadhouders, A. M., De Boer, H., Welling, G. W., Hectors, M. P. C.
Laboratory animals 1985 v.19 no.4 pp. 344-352
Escherichia coli, bile acids, body weight, cecum, feces, flora, host specificity, ileum, laboratory animals, mice, microorganisms, rabbits, volatile fatty acids
Hysterectomy-derived germ-free rabbits were given colonization-resistant caecal flora (CRF) from mice, or microflora obtained from the caecum of an antibiotic-decontaminated conventional rabbit and compared with rabbits conventionally raised with the doe. Bodyweight and the following intestinal parameters were determined for the 3 groups: colonization resistance to E. coli, relative caecal weight, villus:crypt ratio (ileum), β-aspartylglycine (faeces), volatile fatty acids (caecum), and bile acids (faeces). Germ-free rabbits given mouse CRF-flora showed values quite different from control animals (or most parameters, indicating unsuitability of mouse CRF flora to 'normalize' rabbits. In germ-free rabbits given modified (antibiotic-treated) rabbit flora, values for most parameters were intermediate between those found for the other 2 groups. This species-specific caecal flora should be improved to provide full 'normalization' of germ-free rabbits.