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Depletion of sucrose induces changes in the tip growth mechanism of tobacco pollen tubes

Author:
Parrotta, Luigi, Faleri, Claudia, Del Duca, Stefano, Cai, Giampiero
Source:
Annals of botany 2018 v.122 no.1 pp. 23-43
ISSN:
1095-8290
Subject:
1,3-beta-glucan synthase, Nicotiana tabacum, adenosine triphosphate, biochemical pathways, callose, carbon, cell growth, cell walls, culture media, energy, fructose, glucose, glycerol, metabolites, models, pH, pectins, photosynthesis, plant reproduction, pollen tubes, sucrose, sucrose synthase, tobacco
Abstract:
Pollen tubes are rapidly growing, photosynthetically inactive cells that need high rates of energy to support growth. Energy can derive from internal and external storage sources. The lack of carbon sources can cause various problems during pollen tube growth, which in turn could affect the reproduction of plants. We analysed the effects of energy deficiency on the development of Nicotiana tabacum pollen tubes by replacing sucrose with glycerol in the growth medium. We focused on cell growth and related processes, such as metabolite composition and cell wall synthesis. We found that the lack of sucrose affects pollen germination and pollen tube length during a specific growth period. Both sugar metabolism and ATP concentration were affected by sucrose shortage when pollen tubes were grown in glycerol-based media; this was related to decreases in the concentrations of glucose, fructose and UDP-glucose. The intracellular pH and ROS levels also showed a different distribution in pollen tubes grown in sucrose-depleted media. Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase). Furthermore, both callose and newly secreted cell wall material (mainly pectins) showed an altered distribution corresponding to the lack of oscillatory growth in pollen tubes. Growth in glycerol-based media also temporarily affected the movement of generative cells and, in parallel, the deposition of callose plugs. Pollen tubes represent an ideal model system for studying metabolic pathways during the growth of plant cells. In our study, we found evidence that glycerol, a less energetic source for cell growth than sucrose, causes critical changes in cell wall deposition. The evidence that different aspects of pollen tube growth are affected is an indication that pollen tubes adapt to metabolic stress.
Agid:
6245786