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Enhancement of antroquinonol production during batch fermentation using pH control coupled with an oxygen vector

Author:
Xia, Yongjun, Chen, Yan, Liu, Xiaofeng, Zhou, Xuan, Wang, Zhaochu, Wang, Guangqiang, Xiong, Zhiqiang, Ai, Lianzhong
Source:
Journal of the science of food and agriculture 2019 v.99 no.1 pp. 449-456
ISSN:
0022-5142
Subject:
Taiwanofungus camphoratus, anti-inflammatory activity, antineoplastic activity, antroquinonols, batch fermentation, bioreactors, biosynthesis, culture media, dissolved oxygen, kinetics, oxygen, pH, solid state fermentation, submerged fermentation
Abstract:
BACKGROUND: Antroquinonol, a ubiquinone derivative that shows anticancer and anti‐inflammatory activities, is produced during solid‐state fermentation of Antrodia camphorata; however, it cannot be biosynthesized via conventional submerged fermentation. RESULTS: A method for enhancing the biosynthesis of antroquinonol by controlling pH and adding an oxygen vector in a 7 L bioreactor was studied. In shake‐flask experiments, a maximum antroquinonol production of 31.39 ± 0.78 mg L⁻¹ was obtained by fermentation with adding 0.2 g L⁻¹ coenzyme Q₀ (CoQ₀), at the 96th hour. Following kinetic analysis of the fermentation process, pH control strategies were investigated. A maximum antroquinonol production of 86.47 ± 3.65 mg L⁻¹ was achieved when the pH was maintained at 5.0, which exhibited an increase of 348.03% higher than the batch without pH regulation (19.30 ± 0.88 mg L⁻¹). The conversion rate of CoQ₀ improved from 1.51% to 20.20%. Further research revealed that the addition of n‐tetradecane could increase the production of antroquinonol to 115.62 ± 4.87 mg L⁻¹ by increasing the dissolved oxygen in the fermentation broth. CONCLUSION: The results demonstrated that pH played an important role in antroquinonol synthesis in the presence of the effective precursor CoQ₀. It was a very effective strategy to increase the yield of antroquinonol by controlling pH and adding oxygen vector. © 2018 Society of Chemical Industry
Agid:
6245856