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Comparisons of five Saccharomyces cerevisiae strains for ethanol production from SPORL-pretreated lodgepole pine

Zhou, Haifeng, Lan, Tianqing, Dien, Bruce S., Hector, Ronald E., Zhu, J. Y.
Biotechnology progress 2014 v.30 no.5 pp. 1076
Pinus contorta var. latifolia, strains, enzymatic hydrolysis, Saccharomyces cerevisiae, absorbance, biomass, ethanol, ethanol production, fermentation, genetic engineering, genetically engineered microorganisms, hydrolysates, lignocellulose, slurries, starter cultures, toxicity, xylose, yeasts
The performances of 5 yeast strains under three levels of toxicity were evaluated using hydrolysates from lodgepole pine pretreated by Sulfite Pretreatment to Overcome the Recalcitrance of Lignocelluloses (SPORL). The highest level of toxicity was represented by the whole pretreated biomass slurry, while intermediate toxicity was represented by the hydrolysate with partial loading of pretreatment spent liquor. The zero toxicity was represented using the enzymatic hydrolysate made from thoroughly washed SPORL lodgepole pine solids. The results indicate that strains D5A and YRH400 can tolerate the whole pretreated biomass slurry to produce 90.1 and 73.5% theoretical ethanol yield, respectively, at a relatively low yeast loading of optical density of 3.5. Strains Y1528, YRH403, and FPL450 were only able to ferment the hydrolysate with intermediate toxicity with lower ethanol productivity than D5A and YRH400. Both YRH400 and YRH403 were genetically engineered for xylose fermentation but were not able to consume xylose efficiently.