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Simple and effective label free electrochemical immunosensor for Fig mosaic virus detection

Haji-Hashemi, Hedieh, Safarnejad, Mohammad Reza, Norouzi, Parviz, Ebrahimi, Mehrnaz, Shahmirzaie, Morteza, Ganjali, Mohammad Reza
Analytical biochemistry 2019 v.566 pp. 102-106
Fig mosaic virus, antibodies, dielectric spectroscopy, electrochemistry, electrodes, gold, immunosensors, nucleocapsid, polymerase chain reaction, propionic acid, viruses
Here, the construction and characterization of the first immunosensor for highly sensitive and label free detection of Fig mosaic virus (FMV) is reported. The specific antibody against nucleocapsid of the virus was raised and immobilized at the surface of 11-mercaptoundecanoic acid (MUA) and 3-mercapto propionic acid (MPA) modified gold electrode, via carbodiimide coupling reaction. The immunosensor fabrication steps were characterized using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The electrochemical detection of FMV was conducted using differential pulse voltammetry in ferri/ferrocyanide solution as a redox probe. The proposed immunosensor exhibited high selectivity, good reproducibility and high sensitivity for FMV detection in a range from 0.1 nM to 1 μM with a detection limit of 0.03 nM. Moreover, good results were obtained for determination of FMV in real samples, indicating the feasibility of the developed immunosensor for detection of fig mosaic disease, without the need for molecular (e.g. PCR) amplification.