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Interplay of actin, ADP and Mg2+ interactions with striated muscle myosin: Implications of their roles in ATPase

Author:
Kobayashi, Minae, Ramirez, Benjamin E., Warren, Chad M.
Source:
Archives of biochemistry and biophysics 2019 v.662 pp. 101-110
ISSN:
0003-9861
Subject:
actin, adenosine diphosphate, adenosinetriphosphatase, circular dichroism spectroscopy, dissociation, enzyme activity, fluorescence, magnesium, myosin, nuclear magnetic resonance spectroscopy, striated muscle, titration
Abstract:
The effects of Mg2+ on the interaction between ADP, a product of the ATPase reaction, and striated muscle myosin-subfragment 1 (S1) were investigated with both functional and spectroscopic methods. Mg2+ inhibited striated muscle myosin ATPase in the presence of F-actin. Significant effects of Mg2+ were observed in both rate constants of NOE build-up and maximal intensities in WaterLOGSY NMR experiments as F-actin concentration increased. In the absence of F-actin, myosin S1 with Mg2+ bound to a fluorescent ADP analog about five-times tighter than without Mg2+. In the presence of F-actin, the affinity of myosin S1 toward the ADP analog significantly decreased both with and without Mg2+. The equilibrium titration of myosin-S1 into F-actin revealed that in the presence of ADP the apparent dissociation constant (Kd) without Mg2+ was more than five-fold smaller than with Mg2+. Further, we examined effects of F-actin, ADP and Mg2+ binding to myosin on the tertiary structure of myosin-S1 using near UV circular dichroism (CD) spectroscopy. Both in the presence and absence of ADP, there was a Mg2+-dependent difference in the near UV CD spectra of actomyosin. Our results show that Mg2+ affects myosin-ADP and actin-myosin interactions which may be reflected in myosin ATPase activity.
Agid:
6250447