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An in vitro study of the protective effect of caffeic acid on human erythrocytes

Author:
Colina, José R., Suwalsky, Mario, Manrique-Moreno, Marcela, Petit, Karla, Aguilar, Luis F., Jemiola-Rzeminska, Malgorzata, Strzalka, Kazimierz
Source:
Archives of biochemistry and biophysics 2019 v.662 pp. 75-82
ISSN:
0003-9861
Subject:
X-ray diffraction, caffeic acid, differential scanning calorimetry, erythrocyte membrane, erythrocytes, fluorescence emission spectroscopy, humans, in vitro studies, molecular models, phospholipids, protective effect, scanning electron microscopy
Abstract:
The interaction and protective effect of caffeic acid (CA) on human erythrocytes (RBC) and molecular models of its membrane were studied. The latter consisted of bilayers built up of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), representative of phospholipid classes located in the outer and inner monolayers of the human erythrocyte membrane, respectively. X-ray diffraction and differential scanning calorimetry results indicated that CA induced structural and thermotropic perturbations in multilayers and vesicles of DMPC. Fluorescence spectroscopy analysis showed that CA increased the fluidity of DMPC vesicles and of human erythrocyte ghosts. Scanning electron microscopy observations displayed that CA induced morphological alterations to RBC from their normal discoid form to echinocytes. The assessment of its protective capacity showed that CA inhibits RBC morphological alterations and lysis induced by HClO. These findings imply that CA molecules were located in the outer monolayer of the erythrocyte membrane, and that this preferential location might effectively protect the red cells from damage caused by oxidizing species.
Agid:
6250455