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A zebrafish (danio rerio) model for high-throughput screening food and drugs with uric acid-lowering activity

Zhang, Yingyu, Li, Qi, Wang, Fengzhong, Xing, Cencan
Biochemical and biophysical research communications 2019 v.508 no.2 pp. 494-498
Danio rerio, allopurinol, anserine, hyperuricemia, larvae, models, potassium, screening, sodium, uric acid, xanthine
With co-treatment of potassium oxonate (PO) and xanthine sodium salt (XSS), a zebrafish larva model of acute hyperuricemia has been constructed for the first time. The results show PO 200 μM + XSS 10 μM, PO 300 μM + XSS 15 μM, and PO 400 μM + XSS 20 μM can significantly increase the level of uric acid in the zebrafish larvae (P < 0.05), the concentrations as described above can be used to construct the zebrafish larvae model of acute hyperuricemia. At the same time, treatment of allopurinol (APL, one of the hyperuricemia drugs) at 2000 μM (P < 0.001) and treatment of anserine (ASE) at 200 μM (P < 0.05) could significantly decrease the level of uric acid in the model group which received PO 200 μM + XSS 10 μM, which demonstrate that such model could offer a new robust approach for high-throughput screening of food and drugs with uric acid-lowering activity.