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miR-4478 contributes to the radio-sensitivity of ovarian cancer cells by regulating Fus

Ding, Chunli, Hou, Lihui
Biochemical and biophysical research communications 2018
3' untranslated regions, animal ovaries, cell lines, cell proliferation, gain-of-function mutation, irradiation, luciferase, microRNA, mortality, neoplasm cells, non-coding RNA, ovarian neoplasms, patients, relapse, tissues, women
Ovarian cancer is one of the most prevalent cancers among women around the world, with high mortality and relapse rate. MicroRNAs (miRNAs), a class of small non-coding RNAs, have been reported by various studies to regulate multiple biological processes by binding to the 3'UTR of target genes. Radio-resistance is one of the major reasons for treatment failure among ovarian cancer patients. Present study explored the effect of miR-4478/Fus on the cell radio-sensitivity of ovarian cancer. We confirmed the downregulation of miR-4478 and upregulation of Fus in ovarian cancer tissues and cell lines. Kaplan-Meier curve revealed that miR-4478 and Fus were potential prognostic factors for patients with ovarian cancer. The suppressive effect of miR-4478 on ovarian cancer cell proliferation and radio-resistance was uncovered through gain-of-function assays. Mechanism experiments such as luciferase reporter assay and pulldown assay validated the binding of miR-4478 to the 3'UTR of Fus. Spearman's correlation curve revealed the negative expression correlation between miR-4478 and Fus in ovarian cancer tissues. Finally, rescue assays proved that miR-4478 enhanced the sensitivity of ovarian cancer cells to irradiation by interacting with Fus. In summary, present study revealed the role of miR-4478/Fus axis in the cell radio-sensitivity ovarian cancer.